Inhibitory potentials of phenolic-rich extracts from Bridelia ferruginea on two key carbohydrate-metabolizing enzymes and Fe-induced pancreatic oxidative stress / 中西医结合学报

<p><b>OBJECTIVE</b>The current study was designed to evaluate the various antioxidant potentials and inhibitory effects of phenolic-rich leaf extracts of Bridelia ferruginea (BF) on the in vitro activities of some key enzymes involved in the metabolism of carbohydrates.</p><p><b>METHODS</b>In this study, BF leaf free and bound phenolic-rich extracts were used. We quantified total phenolic and flavonoid contents, and evaluated several antioxidant activities using assays for ferric reducing antioxidant power, total antioxidant activity (phosphomolybdenum reducing ability), 1,1-diphenyl-2-picrylhydrazyl and thiobarbituric acid reactive species. Also, extracts were tested for their ability to inhibit α-amylase and α-glucosidase activity.</p><p><b>RESULTS</b>The total phenolic and total flavonoid contents in the free phenolic extract of BF were significantly greater than in the bound phenolic extract. Also, all the antioxidant activities considered were significantly greater in the free phenolic extract than in the bound phenolic extract. In the same vein, the free phenolic-rich extract had a significantly higher percentage inhibition against α-glucosidase activity (IC = 28.5 µg/mL) than the bound phenolic extract (IC = 340.0 µg/mL). On the contrary, the free phenolic extract (IC = 210.0 µg/mL) had significantly lower inhibition against α-amylase than the bound phenolic-rich extract (IC = 190.0 µg/mL).</p><p><b>CONCLUSION</b>The phenolic-rich extracts of BF leaves showed antioxidant potentials and inhibited two key carbohydrate-metabolizing enzymes in vitro.</p>

Evaluation of Anti-diabetic and Liver Enzymes Activity of Aqueous Extracts of Moringa oleifera and Bridelia ferruginea Leaves in Alloxan Induced Diabetic Albino Rats.

To evaluate anti-diabetic and liver enzymes activities of aqueous extracts of Moringa oleifera and Bridelia ferruginea leaves in alloxan induced diabetic albino rats. Study design: Diabetes was induced in three groups of rats, one group was not treated while two groups were treated orally with M. oleifera and B. ferruginea extracts at 200, 400 and 800 mg/kg body weight of rats twice for 1 week respectively. One group was not induced and received distilled water only. The anti-diabetic and liver enzymes activities were determined from blood glucose and transaminases activities of the rats. Place and Duration of Study: Department of Biochemistry Ebonyi State University, Abakaliki, Nigeria, September, 2012. Methodology: Twenty-four albino rats were grouped into A, B, C and D group. Group C and D were further subdivided into C1, C2, C3, D1, D2 and D3, respectively. Diabetes was induced in all the groups, except group A (positive control). Group B (negative control) was not treated while group C and D were treated with aqueous extracts of M. oleifera and B. ferruginea leaves, which were administered orally to the animals twice daily for 1 week at varying concentrations of 200, 400 and 800 mg/kg body weights. The glucose and liver enzymes levels were determined using glucometeric and spectrophotometric methods. Results: The results revealed that there was significant reductions (P < 0.05) in glucose level in rats treated with aqueous extract of B. ferruginea than M. oleifera treated rats. There were no significant reduction (P> 0.05) in Alkaline phosphatase level between the controls and the treated groups, except at 200 mg/kg dose in which Alkaline phosphatase level was high in rats treated with M. oleifera extract. There were significant reduction (P< 0.05) in Alanine aminotransaminase level in rats treated with both M. oleifera and B. ferruginea in comparison to the negative control while there was significant increase (P < 0.05) when compared to the positive control except at 200 mg/kg dose where there was decrease in Alanine aminotransaminase level in both plant extracts. Also there was a significant decrease (P < 0.05) in AST level in rats treated with M. oleifera when compared to controls while there was significant increase (P< 0.05) in Aspartate aminotransaminase level in rats treated with B. ferruginea except at 200 mg/kg where there was decrease when compared to the controls. Conclusion: These suggest that both extracts can be used in ethno-medicine for the management of diabetes mellitus.